Analysis of Hydrophobicity and Antigenicity of Heat Shock Protein 70 from Gwd

Dracunculusmedinensis (a little dragon from Medina) is responsible for Dracunculiasis, popularly known as “guinea worm disease”. The Cyclops is the intermediate host that ingests the larvae of parasite (D.medinensis), that later on ingested by the human from the contaminated stagnant unfiltered water from thewater source. After ingestion these Cyclops are disintegrated by stomach digestive juices and causes the release of the larvae. These larvae travel and penetrate the digestive wall into the body cavity and get entry in abdominal cavity and retroperitoneal space. These larvae mature in adults and soon after the copulation the ovoviviparous female mature and grows in size up to 3m whereas the male dies. After an incubation period of a year these mature female worm comes towards the skin and start formation of a small round bulge on the skin by secreting an irritating chemical which causes severe pain. In order to study the antigenicity and the hydrophobicity of the protein we have taken Heat shock protein 70 (mitochondrion) proteins from D.medinensis, consist of 647aa sequence.The Identification is carried out through Bcell epitopes prediction methods .The results obtained shows that the region of maximal hydrophilicity is likely to be antigenic site having the hydrophobic characteristics and contain the segments of low complexity and high-predicted flexibility. This predicted antigenic protein from D. medinensis promises to play an important role in the further treatment of this disease and target validation in the drug development process.